Anti-Catenin, beta

Beta-catenin is one of the key downstream effectors in the Wnt signaling pathway (1). It has been implicated in two major biological processes of vertebrates: early embryonic development (2) and tumorigenesis (3). GSK-3 beta or other kinases may destabilize beta-catenin by phosphorylating it at serines 33, 37, 45 and threonine 41 (4). Stabilizing mutations of these phosphorylation sites in beta-catenin have been found in many tumor cell lines (5). Applications: Suitable for use in ELISA, Western Blot, Immunoprecipitation and Immunohistochemistry (Paraffin sections). Other applications not tested. , Recommended Dilution:, Western Blot: 1:1000, Immunoprecipitation: 1:200 , Immunohistochemistry (Paraffin): 1:200, Optimal dilutions to be determined by the researcher., , Storage and Stability: May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.