Anti-Brevican (BEHAB, Brain-Enriched Hyaluronan-Binding Protein)

Brain-enriched hyaluronan-binding protein (Brevican) is a 160kD member of the aggregan/versican proteoglycan family of matrix proteins. It is brain-derived and serves as a linker between HA and other matrix molecules such as tenascins and fibulins. Mature human Brevican is 889 amino acids in length. It contains multiple domains, with an N-terminal V-type Ig-like domain (aa52-139) and a C-terminal EGF-like (aa646-682) and C-type lectin domain (aa695-802). Additional isoforms exist resulting from an alternate start site at Met228 and a 23 aa substitution for aa649-911 that serves as a GPI-anchor motif. Over aa23-911, human Brevican shares 82% aa identity with mouse Brevican. Applications: Suitable for use in ELISA, Western Blot, Immunoprecipitation and Inhibition. Other applications not tested. Recommended Dilutions: Western Blot: 1ug/ml under non-reducing conditions only.., Immunoprecipitation: 25ug/ml immunoprecipitates recombinant human Brevican from conditioned cull culture medium., Inhibition (Blocking of receptor-ligand interaction): ~0.01-0.05ug/ml will block 50% of the binding of 1ug/ml of biotinylated Hyaluronan (132kD) to immobilized recombinant human Brevican (100ul of a 3ug/ml solution was coated in each well) in a functional ELISA. 1ug/ml will achieve >90% blocking in the assay., Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized powder may be stored at -20°C. Stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.