Anti-Brevican (BEHAB, Brain-Enriched Hyaluronan-Binding Protein)

Brevican (brain-enriched hyaluronan-binding protein) is a 160kD member of the aggrecan/versican proteoglycan family of matrix proteins. It is brain-derived and serves as a linker between HA and other matrix molecules such as tenascins and fibulins. Mature human Brevican is 889 amino acids in length. It contains multiple domains, with an N-terminal V-type Ig-like domain (aa # 52-139) and a C-terminal EGF-like domain (aa # 646-682) and a C-type lectin domain (aa # 695-802). Multiple isoforms exist where there is an alternate start site at Met228 and a 23 aa substitution for aa # 649-911 that serves as a GPI-anchor motif. Over aa #23-911, human Brevican shares 82% aa identity with mouse Brevican. Applications: Suitable for use in Western Blot and ELISA. Other applications not tested. Recommended Dilution: Western Blot: 0.1-0.2ug/ml, ELISA: 0.5-1ug/ml, Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized powder may be stored at 4°C for short-term only. Reconstitute to nominal volume by adding sterile 40-50% glycerol and store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.