Anti-ATM (Ataxia telangiectasia mutated)

ATM(ataxia telangiectasia mutated),also known as TEL1 or TELO1, is a serine/threonine protein , kinase that is recruited and activated by DNA double-strand breaks. The ATM protein is a member of , the phosphatidylinositol 3-kinase family of proteins that respond to DNA damage by phosphorylating , key substrates involved in DNA repair and/or cell cycle control. Linkage analysis of ataxia-telangiectasia , led to mapping of the ATM gene to chromosome 11q22.3. Using an antiserum developed to a peptide , corresponding to the deduced amino acid sequence of ATM, the ATM protein is a single, high molecular , weight protein predominantly confined to the nucleus of human fibroblasts, although it is present in both , nuclear and microsomal fractions from human lymphoblast cells and peripheral blood , lymphocytes. Overexpression of ATM cDNA in AT cells enhanced their survival after radiation exposure, , decreased radiation-induced chromosome aberrations, reduced radioresistant DNA synthesis, and , partially corrected defective cell cycle checkpoints and induction of stress-activated protein , kinase. ATM has an essential role in the reconstitutive capacity of hematopoietic stem cells but is not , as important for the proliferation or differentiation of progenitors, in a telomere-independent manner. , ATM functions directly in the repair of chromosomal DNA double-stranded breaks by maintaining DNA , ends in repair complexes generated during lymphocyte gene assembly. UniProt Number: Q13315, Gene ID: ATM, Applications: Suitable for use in Western Blot. Recommended Dilution: Optimal dilutions to be determined by the researcher. Storage and Handling: Store at -20?C for one year. After reconstitution, store at 4?C for one month. Can also be aliquoted and stored frozen at -20?C for long term., Avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.