Anti-APC, CT (Adenomatous Polyposis coli)

APC is a ubiquitous, tumor-suppressor protein that is mutated in most colon cancers, generally resulting in a gene product that is truncated in the carboxy terminal region. APC contains several binding domains. The region used as an immunogen overlaps the EB1, DLG (PDZ sequence) and PSD-93 domains. These domains bind microtubules, mitotic spindles and F-actin and are involved in cell cycle, division and migration. The immunogen does not include the beta-catenin-binding region, which antagonizes Wnt signaling. The human APC amino acid sequence used as immunogen is 83% and 86% identical to mouse and rat APC, respectively. Applications: Suitable for use in Western Blot, Immunohistochemistry and ELISA. Optimal dilutions to be determined by the researcher. Recommended Dilutions: Western Blot: 0.1-0.2ug/ml, the detection limit for recombinant human APC C-terminus is~1ng/lane under non-reducing and reducing conditions. , Immunohistochemistry: 5-15ug/ml, ELISA: 0.5-1ug/ml, the detection limit for recombinant human APC C-terminus is ~0.2ng/well., Optimal dilutions to be determined by researcher. Storage and Stability: Lyophilized powder may be stored at -20°C. Stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.