Anti-Ack1-2 (Activated p21cdc42Hs Kinase)

Ack1-2 encodes a tyrosine kinase that binds Cdc42Hs in its GTP-bound form and inhibits both the intrinsic and GTPase-activating protein (GAP)-stimulated GTPase activity of Cdc42Hs. This binding is mediated by a unique sequence of 47aa C-terminal to an SH3 domain. The protein may be involved in a regulatory mechanism that sustains the GTP-bound active form of Cdc42Hs and which is directly linked to a tyrosine phosphorylation signal transduction pathway. Applications: Suitable for use in ELISA and Western Blot. Other applications not tested. Recommended Dilution: Western Blot: 1:500, Optimal dilutions to be determined by the researcher. Recommended Secondary Antibodies: I1906: IgY, Chicken Pab Rb x Ch , I1906-10: IgY, Chicken-HRP Rb x Ch , I1906-12: IgY, Chicken-FITC Rb x Ch , I1906-14: IgY, Chicken-AP Rb x Ch, Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.