Anti-14-3-3 zeta (KCIP-1, YWHAZ)

14­3­3 proteins are a highly conserved family of homo­ and heterodimeric phosphoserine/threonine­binding proteins present in high abundance in all eukaryotic cells. 14­3­3 proteins were the first polypeptides shown to have pSer/Thr binding properties, generally recognizing the consensus sequences RSXpSXP and RXY/FXpSXP (where X is any amino acid). 14­3­3 proteins act as key regulators of intracellular signal transduction through their ability to bind specific motifs phosphorylated on serine or threonine. For example, the binding of 14­3­3 to phosphorylated BAD blocks its proapoptotic association with Bcl­XL. There are at least seven distinct 14­3­3 genes in vertebrates, alpha/beta, epsilon, eta, gamma, theta, sigma and zeta. 14­3­3 zeta, also known as Tyrosine 3­ Monooxygenase/Tryptophan 5­Monooxygenase Activation Protein, zeta isoform (gene name YWHAZ) is a 245 amino acid, 27kD protein. Applications: Suitable for use in ELISA, Western Blot, and Immunohistochemistry. Other applications not tested. Recommended Dilution: Western Blot: 0.2ug/ml, Immunohistochemistry: 8­25ug/ml, Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.