Proto-oncogene Wnt-3 (Wnt3), mouse, recombinant

Organism: Mus musculus (Mouse). Source: E.coli. Expression Region: 22-355aa. Protein Length: Full Length of Mature Protein. Tag Info: N-terminal 10xHis-tagged. Target Protein Sequence: GYPIWWSLAL GQQYTSLASQ PLLCGSIPGL VPKQLRFCRN YIEIMPSVAE GVKLGIQECQ HQFRGRRWNC TTIDDSLAIF GPVLDKATRE SAFVHAIASA GVAFAVTRSC AEGTSTICGC DSHHKGPPGE GWKWGGCSED ADFGVLVSRE FADARENRPD ARSAMNKHNN EAGRTTILDH MHLKCKCHGL SGSCEVKTCW WAQPDFRAIG DFLKDKYDSA SEMVVEKHRE SRGWVETLRA KYALFKPPTE RDLVYYENSP NFCEPNPETG SFGTRDRTCN VTSHGIDGCD LLCCGRGHNT RTEKRKEKCH CVFHWCCYVS CQECIRIYDV HTCK. Purity: Greater than 85% as determined by SDS-PAGE. Endotoxin: Not test. Biological Activity: n/a. Form: Liquid or Lyophilized powder. Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20 °C/-80 °C. Our default final concentration of glycerol is 50%. Customers could use it as reference. Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 °C/-80 °C. The shelf life of lyophilized form is 12 months at -20 °C/-80 °C. Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4 °C for up to one week. Relevance: Ligand for mbers of the frizzled family of seven transmbrane receptors. Wnt-3 and Wnt-3a play distinct roles in cell-cell signaling during morphogenesis of the developing neural tube. Reference: Wnt-3, a gene activated by proviral insertion in mouse mammary tumors, is homologous to int-1/Wnt-1 and is normally expressed in mouse embryos and adult brain.Roelink H., Wagenaar E., Lopes da Silva S., Nusse R.Proc. Natl. Acad. Sci. U.S.A. 87:4519-4523(1990). Function: Ligand for members of the frizzled family of seven transmembrane receptors. Wnt-3 and Wnt-3a play distinct roles in cell-cell signaling during morphogenesis of the developing neural tube.