Protein NOV homolog protein (NOV) (Active), human, recombinant

Organism: Homo sapiens (Human). Source: E.Coli. Expression Region: 28-357aa. Protein Length: Full Length of Mature Protein. Tag Info: Tag-Free. Target Protein Sequence: M+QVAATQRCPP QCPGRCPATP PTCAPGVRAV LDGCSCCLVC ARQRGESCSD LEPCDESSGL YCDRSADPSN QTGICTAVEG DNCVFDGVIY RSGEKFQPSC KFQCTCRDGQ IGCVPRCQLD VLLPEPNCPA PRKVEVPGEC CEKWICGPDE EDSLGGLTLA AYRPEATLGV EVSDSSVNCI EQTTEWTACS KSCGMGFSTR VTNRNRQCEM LKQTRLCMVR PCEQEPEQPT DKKGKKCLRT KKSLKAIHLQ FKNCTSLHTY KPRFCGVCSD GRCCTPHNTK TIQAEFQCSP GQIVKKPVMV IGTCTCHTNC PKNNEAFLQE LELKTTRGKM. Purity: >95% as determined by SDS-PAGE. Endotoxin: Less than 1.0 EU/µg as determined by LAL method. Biological Activity: Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using murine Balb/c 3T3 cells is less than 1.0 µg/ml, corresponding to a specific activity of > 1000 IU/mg. Form: Lyophilized powder. Buffer: Lyophilized from a 0.2 µm filtered PBS, pH 7.0. Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20 °C/-80 °C. Our default final concentration of glycerol is 50%. Customers could use it as reference. Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 °C/-80 °C. The shelf life of lyophilized form is 12 months at -20 °C/-80 °C. Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4 °C for up to one week. Relevance: Immediate-early protein likely to play a role in cell growth regulation. {ECO:0000250}. Reference: n/a. Function: Immediate-early protein playing a role in various cellular processes including proliferation, adhesion, migration, differentiation and survival