CD40 Ligand, active (CD40LG), Recombinant, Human

Mediates B-cell proliferation in the absence of co-stimulus as well as IgE production in the presence of IL-4. Involved in immunoglobulin class switching. {ECO:0000269, PubMed:15193700}. Release of soluble CD40L from platelets is partially regulated by GP IIb/IIIa, actin polymerization, and an matrix metalloproteinases (MMP) inhibitor-sensitive pathway. {ECO:0000269, PubMed:15193700}. Biological Activity: Fully biologically active when compared to standard. The ED50 as determined by the dose-dependent stimulation of IL-8 production by human PBMC is less than 5-10ng/ml. Sequence: MQKGDQNPQI AAHVISEASS KTTSVLQWAE KGYYTMSNNL VTLENGKQLT VKRQGLYYIY AQVTFCSNRE ASSQAPFIAS LWLKSPGRFE RILLRAANTH SSAKPCGQQS IHLGGVFELQ PGASVFVNVT DPSQVSHGTG FTSFGLLKL, Molecular Weight: 16.3kD, PubMed ID: 1280226, 1385114, 7678782, 1281209, 7678552, 7999797, 15489334, 8626375, 11676606, 15193700, 8589998, 9605317, 7679206, 8094231, 7679801, 7717401, 7532185, 8889581, 8550833, 9150729, 9746782, Gene Name: CD40LG, CD40L, TNFSF5, TRAP, Swiss Prot: P29965, Storage and Stability: Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.