Angiopoietin-2 (ANGPT2) (Active), dog, recombinant

Organism: Canis lupus familiaris (Dog) (Canis familiaris). Source: Mammalian cell. Expression Region: 19-495aa. Protein Length: Full Length of Mature Protein. Tag Info: C-terminal 6xHis-tagged. Target Protein Sequence: YNNFRRSMDS IGRRQYQVQH GSCSYTFLLP ETDNCRSPGS YVPNAVQRDA PLDYDDSVQR LQVLENIMEN NTQWLIKLEN YIQDNMKKEM VEMQQNAVQN QTAVMIEIGT NLLNQTAEQT RKLTDVEAQV LNQTTRLELQ LLEHSLSTNK LEKQILDQTS EINKLQDKNS FLEKKVLDME DKHIVQLRSI KEEKDQLQVL VSKQNSIIEE LEKQLVTATV NNSVLQKQQH DLMETVHSLL TMISPSKSPK DTFVAKEEQI IYRDCAEVFK SGLTTNGIYT LTFPNSTEEI KAYCDMETSG GGWTVIQRRE DGSVDFQRTW KEYKVGFGNP SGEHWLGNEF VFQVTNQQPY VLKIHLKDWE GNEAYSLYEH FYLSGEELNY RIHLKGLTGT AGKISSISQP GNDFSTKDAD NDKCICKCSQ MLTGGWWFDA CGPSNLNGMY YPQRQNTNKF NGIKWYYWKG SGYSLKGTTM MIRPADF. Purity: Greater than 95% as determined by SDS-PAGE. Endotoxin: Less than 1.0 EU/ug as determined by LAL method. Biological Activity: Measured by its binding ability in a functional ELISA. Immobilized Dog ANGPT2 at 2 µg/mL can bind anti-ANGPT2 recombinant antibody (CSB-RA001707MA01HU), the EC50 is 2.836-4.992 ng/mL. Form: Lyophilized powder. Buffer: Lyophilized from a 0.2 µm filtered 20 mM Tris-HCl, 0.5 M NaCl, 6% Trehalose, pH 8.0. Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20 °C/-80 °C. Our default final concentration of glycerol is 50%. Customers could use it as reference. Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 °C/-80 °C. The shelf life of lyophilized form is 12 months at -20 °C/-80 °C. Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4 °C for up to one week. Relevance: Binds to TEK/TIE2, competing for the ANGPT1 binding site, and modulating angiogenic signals mediated by ANGPT1. Can induce tyrosine phosphorylation of TEK/TIE2 in the absence of ANGPT1. In the absence of angiogenic inducers, such as VEGF, ANGPT2-mediated loosening of cell-matrix contacts may induce endothelial cell apoptosis with consequent vascular regression. In concert with VEGF, it may facilitate endothelial cell migration and proliferation, thus serving as a permissive angiogenic signal. Reference: n/a. Function: nan