VHL Complex, Active, Recombinant, Human (VCB-Cul2 complex, ECV complex, CBCVHL)

The VHL complex is a multi-subunit ubiquitin ligase composed of VHL, Elongin B, Elongin C, Cul2 and Rbx1. The VHL protein serves as the substrate recognition component and is linked by Elongin C to a heterodimeric Cul2/Rbx1 module that functions as a potent activator of the ubiquitination of target proteins by an E2 conjugating enzyme. Elongin B interacts with the complex through Elongin C and appears to stabilize the binding of Elongin C to VHL. The primary function of the VHL complex is to regulate HIF (hypoxia inducible factor) activity by targeting the hydroxylated HIF-1a subunit for ubiquitination and rapid proteasomal degradation under normoxic conditions. It therefore plays an important role in the regulation of hypoxia-inducible genes such as the vascular endothelial growth factor (VEGF) and glucose transport-1 (Glut-1). Mutations in VHL are associated with the inherited von Hippel-Lindau (VHL) cancer syndrome and numerous forms of renal cell carcinoma. Recombinant protein corresponding to aa54-end from human VHL, fused to GST-tag at N-terminus, human Elongin C, GST-tag at N-terminus, human Elongin B, human Cul2, fused to 6His tagged at N-terminal, and human Rbx1, co-expressed by baculovirus in Sf21 insect cells. , Molecular Weight: , VHL: ~45kD, Elongin C: ~40kD, Elongin B: ~13kD, Cul2: ~91kD, Rbx1: ~12kD, Storage and Stability: Aliquot to avoid repeated freezing and thawing and store at -70°C. Aliquots are stable for 6 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.