Sphingomyelin phosphodiesterase 3 (SMPD3), partial, human, recombinant

Organism: Homo sapiens (Human). Source: E.coli. Expression Region: 86-655aa. Protein Length: Partial. Tag Info: N-terminal 10xHis-tagged and C-terminal Myc-tagged. Target Protein Sequence: SPLQSARRPY IYSRLEDKGL AGGAALLSEW KGTGPGKSFC FATANVCLLP DSLARVNNLF NTQARAKEIG QRIRNGAARP QIKIYIDSPT NTSISAASFS SLVSPQGGDG VARAVPGSIK RTASVEYKGD GGRHPGDEAA NGPASGDPVD SSSPEDACIV RIGGEEGGRP PEADDPVPGG QARNGAGGGP RGQTPNHNQQ DGDSGSLGSP SASRESLVKG RAGPDTSASG EPGANSKLLY KASVVKKAAA RRRRHPDEAF DHEVSAFFPA NLDFLCLQEV FDKRAATKLK EQLHGYFEYI LYDVGVYGCQ GCCSFKCLNS GLLFASRYPI MDVAYHCYPN KCNDDALASK GALFLKVQVG STPQDQRIVG YIACTHLHAP QEDSAIRCGQ LDLLQDWLAD FRKSTSSSSA ANPEELVAFD VVCGDFNFDN CSSDDKLEQQ HSLFTHYRDP CRLGPGEEKP WAIGTLLDTN GLYDEDVCTP DNLQKVLESE EGRREYLAFP TSKSSGQKGR KELLKGNGRR IDYMLHAEEG LCPDWKAEVE EFSFITQLSG LTDHLPVAMR LMVSSGEEEA. Purity: Greater than 90% as determined by SDS-PAGE. Endotoxin: Not test. Biological Activity: n/a. Form: Liquid or Lyophilized powder. Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20 °C/-80 °C. Our default final concentration of glycerol is 50%. Customers could use it as reference. Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 °C/-80 °C. The shelf life of lyophilized form is 12 months at -20 °C/-80 °C. Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4 °C for up to one week. Relevance: Catalyzes the hydrolysis of sphingomyelin to form ceramide and phosphocholine. Ceramide mediates numerous cellular functions, such as apoptosis and growth arrest, and is capable of regulating these 2 cellular events independently. Also hydrolyzes sphingosylphosphocholine. Regulates the cell cycle by acting as a growth suppressor in confluent cells. Probably acts as a regulator of postnatal development and participates in bone and dentin mineralization. Binds to anionic phospholipids (APLs) such as phosphatidylserine (PS) and phosphatidic acid (PA) that modulate enzymatic activity and subcellular location. May be involved in IL-1-beta-induced JNK activation in hepatocytes. May act as a mediator in transcriptional regulation of NOS2/iNOS via the NF-kappa-B activation under inflammatory conditions. Reference: "Role for mammalian neutral sphingomyelinase 2 in confluence-induced growth arrest of MCF7 cells." Marchesini N., Osta W., Bielawski J., Luberto C., Obeid L.M., Hannun Y.A. J. Biol. Chem. 279:25101-25111(2004). Function: nan