Sarcoplasmic/endoplasmic reticulum calcium ATPase 2 (ATP2A2), partial, human, recombinant

Organism: Homo sapiens (Human). Source: E.coli. Expression Region: 314-756aa. Protein Length: Partial. Tag Info: C-terminal 6xHis-tagged. Target Protein Sequence: VITTCLALGT RRMAKKNAIV RSLPSVETLG CTSVICSDKT GTLTTNQMSV CRMFILDRVE GDTCSLNEFT ITGSTYAPIG EVHKDDKPVN CHQYDGLVEL ATICALCNDS ALDYNEAKGV YEKVGEATET ALTCLVEKMN VFDTELKGLS KIERANACNS VIKQLMKKEF TLEFSRDRKS MSVYCTPNKP SRTSMSKMFV KGAPEGVIDR CTHIRVGSTK VPMTSGVKQK IMSVIREWGS GSDTLRCLAL ATHDNPLRRE EMHLEDSANF IKYETNLTFV GCVGMLDPPR IEVASSVKLC RQAGIRVIMI TGDNKGTAVA ICRRIGIFGQ DEDVTSKAFT GREFDELNPS AQRDACLNAR CFARVEPSHK SKIVEFLQSF DEITAMTGDG VNDAPALKKA EIGIAMGSGT AVAKTASEMV LADDNFSTIV AAVEEGRAIY NNM. Purity: Greater than 95% as determined by SDS-PAGE. Endotoxin: n/a. Biological Activity: n/a. Form: Liquid or Lyophilized powder. Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20 °C/-80 °C. Our default final concentration of glycerol is 50%. Customers could use it as reference. Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 °C/-80 °C. The shelf life of lyophilized form is 12 months at -20 °C/-80 °C. Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4 °C for up to one week. Relevance: This magnesium-dependent enzyme catalyzes the hydrolysis of ATP coupled with the translocation of calcium from the cytosol to the sarcoplasmic reticulum lumen. Involved in autophagy in response to starvation. Upon interaction with VMP1 and activation, controls ER-isolation membrane contacts for autophagosome formation. Also modulates ER contacts with lipid droplets, mitochondria and endosomes. , [Isoform 2]: Involved in the regulation of the contraction/relaxation cycle. Acts as a regulator of TNFSF11-mediated Ca(2+) signaling pathways via its interaction with TMEM64 which is critical for the TNFSF11-induced CREB1 activation and mitochondrial ROS generation necessary for proper osteoclast generation. Association between TMEM64 and SERCA2 in the ER leads to cytosolic Ca(2+) spiking for activation of NFATC1 and production of mitochondrial ROS, thereby triggering Ca(2+) signaling cascades that promote osteoclast differentiation and activation. Reference: n/a. Function: nan