High affinity cGMP-specific 3,5-cyclic phosphodiesterase 9A (Pde9a), mouse, recombinant

Organism: Mus musculus (Mouse). Source: E.coli. Expression Region: 1-534aa. Protein Length: Full Length. Tag Info: N-terminal 6xHis-tagged. Target Protein Sequence: MGAGSSSYRP KAIYLDIDGR IQKVVFSKYC NSSDIMDLFC IATGLPRNTT ISLLTTDDAM VSIDPTMPAN SERTPYKVRP VAVKQVSERE ELIQGVLAQV AEQFSRAFKI NELKAEVANH LAVLEKRVEL EGLKVVEIEK CKSDIKKMRE ELAARNSRTN CPCKYSFLDN KKLTPRRDVP TYPKYLLSPE TIEALRKPTF DVWLWEPNEM LSCLEHMYHD LGLVRDFSIN PITLRRWLLC VHDNYRNNPF HNFRHCFCVT QMMYSMVWLC GLQEKFSQMD ILVLMTAAIC HDLDHPGYNN TYQINARTEL AVRYNDISPL ENHHCAIAFQ ILARPECNIF ASVPPEGFRQ IRQGMITLIL ATDMARHAEI MDSFKEKMEN FDYSNEEHLT LLKMILIKCC DISNEVRPME VAEPWVDCLL EEYFMQSDRE KSEGLPVAPF MDRDKVTKAT AQIGFIKFVL IPMFETVTKL FPVVEETMLR PLWESREHYE ELKQLDDAMK ELQKKTESLT SGAPENTTEK NRDAKDSEGH SPPN. Purity: Greater than 85% as determined by SDS-PAGE. Endotoxin: Not test. Biological Activity: n/a. Form: Liquid or Lyophilized powder. Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20 °C/-80 °C. Our default final concentration of glycerol is 50%. Customers could use it as reference. Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 °C/-80 °C. The shelf life of lyophilized form is 12 months at -20 °C/-80 °C. Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4 °C for up to one week. Relevance: Specifically hydrolyzes the second messenger cGMP, which is a key regulator of many important physiological processes (PubMed:9624145). Highly specific: compared to other members of the cyclic nucleotide phosphodiesterase family, has the highest affinity and selectivity for cGMP. Specifically regulates natriuretic-peptide-dependent cGMP signaling in heart, acting as a regulator of cardiac hypertrophy in myocytes and muscle. Does not regulate nitric oxide-dependent cGMP in heart (PubMed:25799991). Additional experiments are required to confirm whether its ability to hydrolyze natriuretic-peptide-dependent cGMP is specific to heart or is a general feature of the protein (Probable). In brain, involved in cognitive function, such as learning and long-term memory (PubMed:22328573, PubMed:24746365). Reference: "Phosphodiesterase 9A controls nitric-oxide-independent cGMP and hypertrophic heart disease." Lee D.I., Zhu G., Sasaki T., Cho G.S., Hamdani N., Holewinski R., Jo S.H., Danner T., Zhang M., Rainer P.P., Bedja D., Kirk J.A., Ranek M.J., Dostmann W.R., Kwon C., Margulies K.B., Van Eyk J.E., Paulus W.J., Takimoto E., Kass D.A. Nature 519:472-476(2015). Function: nan