Cytochrome P450 2C19 (CYP2C19), human, recombinant

Organism: Homo sapiens (Human). Source: E.coli. Expression Region: 26-490aa. Protein Length: Full Length of Mature Protein. Tag Info: N-terminal 10xHis-tagged and C-terminal Myc-tagged. Target Protein Sequence: RGKLPPGPTP LPVIGNILQI DIKDVSKSLT NLSKIYGPVF TLYFGLERMV VLHGYEVVKE ALIDLGEEFS GRGHFPLAER ANRGFGIVFS NGKRWKEIRR FSLMTLRNFG MGKRSIEDRV QEEARCLVEE LRKTKASPCD PTFILGCAPC NVICSIIFQK RFDYKDQQFL NLMEKLNENI RIVSTPWIQI CNNFPTIIDY FPGTHNKLLK NLAFMESDIL EKVKEHQESM DINNPRDFID CFLIKMEKEK QNQQSEFTIE NLVITAADLL GAGTETTSTT LRYALLLLLK HPEVTAKVQE EIERVVGRNR SPCMQDRGHM PYTDAVVHEV QRYIDLIPTS LPHAVTCDVK FRNYLIPKGT TILTSLTSVL HDNKEFPNPE MFDPRHFLDE GGNFKKSNYF MPFSAGKRIC VGEGLARMEL FLFLTFILQN FNLKSLIDPK DLDTTPVVNG FASVPPFYQL CFIPV. Purity: Greater than 85% as determined by SDS-PAGE. Endotoxin: Not test. Biological Activity: n/a. Form: Liquid or Lyophilized powder. Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20 °C/-80 °C. Our default final concentration of glycerol is 50%. Customers could use it as reference. Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 °C/-80 °C. The shelf life of lyophilized form is 12 months at -20 °C/-80 °C. Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4 °C for up to one week. Relevance: Responsible for the metabolism of a number of therapeutic agents such as the anticonvulsant drug S-mephenytoin, omeprazole, proguanil, certain barbiturates, diazepam, propranolol, citalopram and imipramine. Oxygenates (R)- and (S)-limonene to produce carveol and perillyl alcohol. Hydroxylates fenbendazole at the 4' position. Reference: "Isolation and characterization of human liver cytochrome P450 2C19: correlation between 2C19 and S-mephenytoin 4'-hydroxylation." Wrighton S.A., Stevens J.C., Becker G.W., VandenBranden M. Arch. Biochem. Biophys. 306:240-245(1993). Function: nan