Cytochrome P450 11B2, mitochondrial (CYP11B2), human, recombinant

Organism: Homo sapiens (Human). Source: Yeast. Expression Region: 25-503aa. Protein Length: Full Length of Mature Protein. Tag Info: N-terminal 6xHis-tagged. Target Protein Sequence: GTRAARAPRT VLPFEAMPQH PGNRWLRLLQ IWREQGYEHL HLEMHQTFQE LGPIFRYNLG GPRMVCVMLP EDVEKLQQVD SLHPCRMILE PWVAYRQHRG HKCGVFLLNG PEWRFNRLRL NPDVLSPKAV QRFLPMVDAV ARDFSQALKK KVLQNARGSL TLDVQPSIFH YTIEASNLAL FGERLGLVGH SPSSASLNFL HALEVMFKST VQLMFMPRSL SRWISPKVWK EHFEAWDCIF QYGDNCIQKI YQELAFNRPQ HYTGIVAELL LKAELSLEAI KANSMELTAG SVDTTAFPLL MTLFELARNP DVQQILRQES LAAAASISEH PQKATTELPL LRAALKETLR LYPVGLFLER VVSSDLVLQN YHIPAGTLVQ VFLYSLGRNA ALFPRPERYN PQRWLDIRGS GRNFHHVPFG FGMRQCLGRR LAEAEMLLLL HHVLKHFLVE TLTQEDIKMV YSFILRPGTS PLLTFRAIN. Purity: Greater than 90% as determined by SDS-PAGE. Endotoxin: Not test. Biological Activity: n/a. Form: Liquid or Lyophilized powder. Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20 °C/-80 °C. Our default final concentration of glycerol is 50%. Customers could use it as reference. Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 °C/-80 °C. The shelf life of lyophilized form is 12 months at -20 °C/-80 °C. Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4 °C for up to one week. Relevance: Preferentially catalyzes the conversion of 11-deoxycorticosterone to aldosterone via corticosterone and 18-hydroxycorticosterone. Reference: "Congenitally defective aldosterone biosynthesis in humans: the involvement of point mutations of the P-450C18 gene (CYP11B2) in CMO II deficient patients." Mitsuuchi Y., Kawamoto T., Naiki Y., Miyahara K., Toda K., Kuribayashi I., Orii T., Yasuda K., Miura K., Nakao K., Imura H., Ulick S., Shizuta Y. Biochem. Biophys. Res. Commun. 182:974-979(1992). Function: Preferentially catalyzes the conversion of 11-deoxycorticosterone to aldosterone via corticosterone and 18-hydroxycorticosterone.