HCMVA (IE2) Peptide Pool

Pool of 143 overlapping peptides derived from a peptide scan (15mers with 11 aa overlap) through 45 kDa immediate-early protein 2 (IE-2) (UniProt ID: P19893) of Human cytomegalovirus (HHV-5) frequently used as positive control One unit allows the stimulation of 2,5 x 10^8 cells. Protein function: Stimulates viral early and late gene expression and thus play a crucial role in the regulation of productive infection (PubMed:31371453). Selectively drives host RNA Pol II transcription initiation at a subset of viral early-late and late promoters without substantially affecting Pol II transcription of expressed host genes. Mechanistically, forms a repressive complex at the major immediate- early promoter region involving direct association with host nucleosomes and TBP. Concerning activation, stimulates transcription by binding nearby, but not within, core promoter regions. In addition, activates quiescent cells to reenter the cell cycle and up-regulates several E2F-responsive genes, which are responsible for pushing the cell into S phase (PubMed:10516036). In S-phase, inhibits cellular DNA synthesis and blocks further cell cycle progression. [The UniProt Consortium]