EBV (LMP2) Peptide Pool

Pool of 122 overlapping peptides derived from a peptide scan (15mers with 11 aa overlap) through Latent membrane protein 2 (LMP2) (UniProt ID: P13285) of Epstein-Barr virus (HHV4) for T cell assays. One unit allows the stimulation of 2,5 x 10^8 cells. Protein function: Isoform LMP2A maintains EBV latent infection of B-lymphocyte, by preventing lytic reactivation of the virus in response to surface immunoglobulin (sIg) cross-linking. Acts like a dominant negative inhibitor of the sIg-associated protein tyrosine kinases, LYN and SYK. Also blocks translocation of the B-cell antigen receptor (BCR) into lipid rafts, preventing the subsequent signaling and accelerated internalization of the BCR upon BCR cross-linking. Serves as a molecular scaffold to recruit SYK, LYN and E3 protein-ubiquitin ligases, such as ITCH and NEDD4L, leading to ubiquitination and potential degradation of both tyrosines kinases. Possesses a constitutive signaling activity in non-transformed cells, inducing bypass of normal B lymphocyte developmental checkpoints allowing immunoglobulin-negative cells to colonize peripheral lymphoid organs. [The UniProt Consortium]