Neurofilament-L Blocking Peptide

Peptide is used to block Neurofilament-L Rabbit mAb reactivity in immunohistochemistry protocols. The cytoskeleton consists of three types of cytosolic fibers: actin microfilaments, intermediate filaments and microtubules. Neurofilaments are the major intermediate filaments found in neurons and consist of light (NFL), medium (NFM) and heavy (NFH) subunits (1). Similar in structure to other intermediate filament proteins, neurofilaments have a globular amino-terminal head, a central alpha-helical rod domain and a carboxy-terminal tail. A heterotetrameric unit (NFL-NFM and NFL-NFH) forms a protofilament, with eight protofilaments comprising the typical 10 nm intermediate filament (2). Neurofilaments are critical for radial axon growth and determine axon caliber while microtubules are involved in axon elongation. PKA phosphorylates the head domain of NFL and NFM to inhibit neurofilament assembly (3-4). Accumulation of neurofilaments are found in many human neurological disorders including Parkinson disease (in Lewy bodies along with alpha-synuclein), Alzheimer disease, Charcot-Marie-Tooth disease and Amyotrophic Lateral Sclerosis (ALS) (1). Quality Control: The quality of the peptide was evaluated by reversed-phase HPLC and by mass spectrometry. The peptide blocks Neurofilament-L Rabbit mAb by immunohistochemistry. , Quality Control: The quality of the peptide was evaluated by reversed-phase HPLC and by mass spectrometry. The peptide blocks Neurofilament-L Rabbit mAb by immunohistochemistry. , , Directions For Use: For immunohistochemistry, add twice the volume of peptide as volume of antibody used in 100ul total volume. Incubate for a minimum of 30 minutes prior to adding the entire volume to the slide. Recommended antibody dilutions can be found on the relevant product data sheet. Storage and Stability: May be stored at 4°C for short-term only. For long-term storage, aliquot and store at -20°C. Aliquots are stable for at least 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.