IKK gamma, NT, Blocking Peptide (IKK3, NEMO, FIP3, IKKAP1, IkappaB Kinase gamma, IKKg), Purified

Peptide (CQYQAPDMDTLQIHVME) corresponding to amino acids 400 to 416 of human IKKg (1,2), which are identical to those of mouse homologue (2). Nuclear factor kappa B (NF-kB) is a ubiquitous transcription factor and an essential mediator of gene expression during activation of immune and in?ammatory responses. NF-kB mediates the expression of a great variety of genes in response to extracellular stimuli. NF-kB is associated with IkB proteins in the cell cytoplasm, which inhibit NF-kB activity. The IkB kinase (IKKa and IKKb) phosphorylates IkB and mediates NF-kB activation. A novel molecule in the IKK complex was recently identi?ed and termed IKKg/NEMO/FIP3/IKKAP1 (1-5). IKKg interacts with IKKb and is required for the activation of IKK complex and NF-kB by LPS, PMA, TNF, and IL-1 stimulation (1-4). FIP3 was also shown to bind to RIP and NIK and to mediate TNF-induced NF-kB activation(3). , Applications: Suitable for use in ELISA and Western Blot. Other applications not tested., , Storage and Stability: Lyophilized powder may be stored at -20°C. Reconstitute to nominal volume by adding sterile 25% glycerol and store at -20°C. May be stored at 4°C for short-term only. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.