Acinus, Blocking Peptide, Purified

Peptide (TRTALHGVKWPQSNPK) corresponding to amino acids 1065-1080 of human AcinusL, 338-353 of human AcinusS', or 307-322 of human AcinusS, which are identical to those of mouse Acinus (2). The selected antigenic sequence is located near the C-terminus of the cleaved active peptide p17. For use with cat. no. A0600., , Chromatin condensation and nuclear fragmentation (CCNF) is the hallmark of apoptosis. CCNF is triggered by the activation of members of caspase family, caspase activated DNase (CAD/DFF40), and several novel proteins including AIF and CIDE (1). A new inducer of chromatin condensation was recently identified and designated Acinus (for apoptotic chromatin condensation inducer in the nucleus). Acinus is cleaved by caspase-3 and an additional unknown protease generating a small active peptide p17, which causes chromatin condensation in vitro when it is added to purified nuclei. Acinus also induces apoptotic chromatin condensation in cells. Acinus is ubiquitously expressed. Three different spliced forms of Acinus have been identified in human and mouse and designated AcinusL, AcinusS and AcinusS' (2).