Z-FA-FMK

Z-FA-FMK is an inhibitor of cathepsin B (Ki = 1. µM). It also is an inhibitor of caspase-2, -3, -6, -7, and -9 (IC50 = 6. 15. 32. 9. and 110. µM, respectively). Z-FA-FMK inhibits severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) main protease (Mpro), also known as 3C-like protease (3CLpro), in a cell-free assay (IC50 = 11. µM). It inhibits SARS-CoV-2 replication in infected Vero E6 cells (EC50 = 0. µM) without inducing cytotoxicity in the same cells with a 50% cytotoxic concentration (CC50) value of >20 µM. Z-FA-FMK (50 µM) decreases the proliferation of, and levels of glutathione (GSH) in, and increases levels of reactive oxygen species (ROS) in anti-CD3-stimulated primary human peripheral blood mononuclear cells (PBMCs). It inhibits LPS-induced increases in levels of IL-1beta in PU5-1. macrophages and Nf-kappaB transactivation in Mf4/4 macrophages when used at a concentration of 50 µM.. Solulibility: DMSO: soluble: .