SR 4835

SR 4835 is an inhibitor of cyclin-dependent kinase 12 (Cdk12) and Cdk13. It binds to Cdk12 and Cdk13 (Kds = 98 and 4.9 nM, respectively) and inhibits Cdk12- and Cdk13-dependent phosphorylation of RNA polymerase II in MDA-MB-231 cells (EC50 = 105.5 nM). SR 4835 inhibits the proliferation of MDA-MB-231, MDA-MB-468, Hs 578T, and MDA-MB-436 breast cancer cells (EC50s = 15.5, 22.1, 19.9, and 24.9 nM, respectively). It induces the DNA damage response and apoptosis in MDA-MB-231 cells when used at concentrations ranging from 30 to 90 nM. In vivo, SR 4835 (20 mg/kg) reduces tumor volume and decreases the number of mice with tumors greater than 1,000 mm3 in a patient-derived xenograft (PDX) model of breast cancer.Formal Name: N-[(5,6-dichloro-1H-benzimidazol-2-yl)methyl]-9-(1-methyl-1H-pyrazol-4-yl)-2-(4-morpholinyl)-9H-purin-6-amine. CAS Number: 2387704-62-1. Molecular Formula: C21H20Cl2N10O. Formula Weight: 499.4. Purity: >98%. Formulation: (Request formulation change), A solid. Solubility: DMSO: Soluble, Methanol: Soluble. lambdamax: 212, 293, 299 nm. SMILES: ClC1=CC2=C(C=C1Cl)NC(CNC3=NC(N4CCOCC4)=NC5=C3N=CN5C6=CN(C)N=C6)=N2. InChi Code: InChI=1S/C21H20Cl2N10O/c1-31-10-12(8-26-31)33-11-25-18-19(29-21(30-20(18)33)32-2-4-34-5-3-32)24-9-17-27-15-6-13(22)14(23)7-16(15)28-17/h6-8,10-11H,2-5,9H2,1H3,(H,27,28)(H,24,29,30). InChi Key: FSELUFUYNUNZKD-UHFFFAOYSA-N.