Poloxin

Poloxin is an inhibitor of the polo-like kinase 1 (Plk1) polo-box domain (PBD, apparent IC50 = 4.8 µM). It is selective for the Plk1 PBD over Plk2 and Plk3 PBDs (IC50s = 18.7 and 53.9 µM, respectively). Poloxin (5, 10, and 25 µM) induces cell cycle arrest at the G2/M phase, apoptosis, and mislocalization of Plk1 in HeLa cells. It reduces cell proliferation in a panel of 10 cancer cell lines (EC50s = 15-35 µM). In vivo, poloxin (40 mg/kg) reduces tumor growth and induces intratumor apoptosis in an MDA-MB-231 breast cancer mouse xenograft model.Formal Name: 1-[O-(2-methylbenzoyl)oxime]2-methyl-5-(1-methylethyl)-2,5-cyclohexadiene-1,4-dione. CAS Number: 321688-88-4. Molecular Formula: C18H19NO3. Formula Weight: 297.4. Purity: >98%. Formulation: (Request formulation change), A solid. Solubility: DMF: 1 mg/ml, DMSO: 2 mg/ml, Ethanol: 1 mg/ml, PBS (pH 7.2): insol. lambdamax: 290 nm. SMILES: O=C(C1=C(C)C=CC=C1)ON=C2C=C(C(C)C)C(C=C2C)=O. InChi Code: InChI=1S/C18H19NO3/c1-11(2)15-10-16(13(4)9-17(15)20)19-22-18(21)14-8-6-5-7-12(14)3/h5-11H,1-4H3. InChi Key: CMOJHDQJJPIVEC-UHFFFAOYSA-N.