NSC 617145

NSC 617145 is an inhibitor of WRN helicase (IC50 = 230 nM) that inhibits the ATPase, but not exonuclease, activity of WRN helicase in a concentration-dependent manner. It is selective for WRN over BLM, FANCJ, ChlR1, RecQ, and UvrD helicases, only exhibiting 7% inhibition of RECQ1 at a concentration of 5 µM. NSC 617145 inhibits growth of HeLa cells via formation of DNA double strand breaks (DSBs) and induction of apoptosis in a WRN helicase-dependent manner. NSC 617145 also acts synergistically with mitomycin C (Cay-11435) to inhibit growth as well as induce DSBs and chromosomal abnormalities in Fanconia-anemia deficient (FA-D2-/-) cells.Formal Name: 1,1'-(2,2-dimethyl-1,3-propanediyl)bis[3,4-dichloro-1H-pyrrole-2,5-dione. CAS Number: 203115-63-3. Molecular Formula: C13H10Cl4N2O4. Formula Weight: 400.0. Purity: >98%. Formulation: (Request formulation change), A crystalline solid. Solubility: DMF: 2 mg/ml, DMF:PBS (pH 7.2) (1:2): 0.33 mg/ml, DMSO: 1 mg/ml. lambdamax: 240 nm. SMILES: CC(CN(C(C(Cl)=C1Cl)=O)C1=O)(C)CN2C(C(Cl)=C(Cl)C2=O)=O. InChi Code: InChI=1S/C13H10Cl4N2O4/c1-13(2,3-18-9(20)5(14)6(15)10(18)21)4-19-11(22)7(16)8(17)12(19)23/h3-4H2,1-2H3. InChi Key: PCOXPBOKDABARQ-UHFFFAOYSA-N.