Indirubin-3'-monoxime

Indirubin-3'-oxime is a potent inhibitor of glycogen synthase kinase 3beta (GSK3beta, IC50 = 22 nM). As GSK3beta phosphorylates tau protein, indirubin-3'-oxime prevents tau phosphorylation both in vitro and in vivo at Alzheimer's disease-relevant sites. It also inhibits cyclin-dependent kinases (CDKs) at higher concentrations, including Cdk1/cyclin B (IC50 = 180 nM), Cdk2/cyclin A (IC50 ~500 nM), Cdk2/cyclin E (IC50 = 250nM), Cdk4/cyclin D1 (IC50 = 3.3 µM) and Cdk5/p35 (IC50 = 100 nM). Indirubin-3'-oxime reversibly inhibits the proliferation of many cells types, arresting cycling in the G2/M phase.Formal Name: 3-[1,3-dihydro-3-(hydroxyimino)-2H-indol-2-ylidene]-1,3-dihydro-2H-indol-2-one. CAS Number: 160807-49-8. Molecular Formula: C16H11N3O2. Formula Weight: 277.3. Purity: >98%. Formulation: (Request formulation change), A crystalline solid. Solubility: DMF: 10 mg/ml, DMSO: 10 mg/ml, DMSO:PBS (pH 7.2) (1:5): 0.15 mg/ml, Ethanol: 2 mg/ml. lambdamax: 212, 252, 280, 286, 328, 342, 502 nm. SMILES: O/N=C1\C(=C2/C(=O)Nc3ccccc\23)/Nc2ccccc/12. InChi Code: InChI=1S/C16H11N3O2/c20-16-13(9-5-1-3-7-11(9)18-16)15-14(19-21)10-6-2-4-8-12(10)17-15/h1-8,17,21H,(H,18,20)/b15-13-,19-14+. InChi Key: HBDSHCUSXQATPO-BGBJRWHRSA-N.