Ampicillin Sodium Salt (6-[D-(-)-alpha-aminophenylacetamido]-penicillanic acid sodium salt)

Ampicillin Sodium Salt is a readily-soluble form of ampicillin. It belongs to the group of beta-lactam antibiotics. These antibotics gram-negative bacteria to which E. coli and Salmonella belong. beta-lactam antibiotic prevent the formation of peptidoglycan, an essential building block of the cell membrane. Thus, preventing growth of cells. Used to select for drug-resistant, plasmid-bearing bacteria. Synonyms: 6-[D-(-)-alpha-Aminophenylacetamido]-penicillanic acid, sodium salt, Sodium [2S-[2alpha,5alpha,6beta (S*)]]-6-(aminophenylacetamido)-3,3-dimethyl-7-oxo-4-thia-1-azabicyclo[3.2.0]heptane-2-carboxylate, CAS No: 69-52-3, Molecular Formula: C16H18N3O4SNa, Molecular Weight: 371.40, Appearance: White to off-white powder, Purity: >98%, Potency: 845-988ug/mg, Identification: To Pass USP, pH (1%): 8.0-10.0, Water: <2%, Methylene Chloride: <0.2%, Solubility (50mg/ml, H2O): Very soluble in water, isotonic sodium chloride and dextrose solutions. Clear, colorless and complete. Insoluble in alcohol, chloroform, ether and fixed oils but soluble in dilute acids or bases. The solution should not be autoclaved, a stock solution should be sterilized through filtration and stored frozen, where it will be stable for months. Antimicrobial Spectrum: Includes both gram-positive (similar to benzylpenicillin) and gram-negative bacteria (similar to tetracyclines and chloramphenicol. Working Concentration: 20-125ug/ml for E. coli in ampicillin-resistance studies, 100mg/L for antibacterial use in cell culture media, Storage and Stablility: Lyophilized powder may be stored at 4°C. Stable for 12 months after receipt at 4°C. Reconstitute with sterile buffer or ddH2O. Aliquot and store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.