Cell Meter(TM) Cellular Senescence Activity Assay Kit

Cellular Senescence is an irreversible growth arrest triggered in order to prevent growth in DNA damaged cells. Senescence-associated beta-galactosidase (SA-beta-gal) is highly overexpressed in senescent cells and it has been widely used as a senescence marker. X-gal staining, a colorimetric method is widely available and used to detect SA-beta-gal in senescent cells. The color method has some limitations such as requirement of fixation of samples due to the low cell permeability of X-gal, longer staining time and low sensitivity. Cell Meter(TM) Cellular Senescence Activity Assay Kit uses Xite(TM) beta-D-galactopyranoside, a fluorogenic beta-Gal substrate that readily enters into live cells, and gets cleaved by SA-beta-gal inside cells, generating strong green fluorescence. Unlike cell-impermeable X-Gal substrate, it has excellent cell permeability. Cell Meter(TM) Cellular Senescence Activity Assay Kit enables users to detect the senescence with higher sensitivity with robust performance. The Xite product is well retained inside the cells, producing a stable signal for fluorescence imaging and flow cytometry analysis.