PARP2 Homogenous Assay Kit

The PARP2 Homogeneous Assay Kit is designed to measure PARP2 activity for screening and profiling applications. PARP2 is known to catalyze the NAD-dependent addition of poly(ADP-ribose) to histones. The PARP2 Homogeneous Assay Kit takes advantage of a highly specific antibody that recognizes the PARylated form of the substrate. With this kit, only three simple steps are required for PARP2 reactions. First, a sample containing PARP2 enzyme is incubated with biotinylated substrate and NAD for one hour. Next, acceptor beads and primary antibody are added, then donor beads, followed by reading the Alpha-counts.