KRAS Isoform B Coupled Nucleotide Exchange (TR-FRET) Assay Kit

The KRAS Isoform B Coupled Nucleotide Exchange TR-FRET Assay Kit is a TR-FRET (Time-Resolved Fluorescence Resonance Energy Transfer) assay kit designed for screening and profiling of KRAS Isoform B antagonists/inhibitors. It monitors the binding of an effector protein such as the Ras binding domain of RAF1, (RBD-cRaf) to KRAS. The KRAS Isoform B Coupled Nucleotide Exchange TR-FRET Assay Kit comes in a convenient 384-well format, with enough purified recombinant GDP-loaded KRAS Isoform B, GTP, exchange factor SOS1 (son of sevenless homolog 1) (amino acids 564-1049), an effector protein RBD-cRAF (amino acids 50-140), assay buffer and additives for 400 reactions.