Anti-phospho-Protein Kinase C Substrate (Ser) (PKC)

Although protein kinase C (PKC) family members are involved in a number of cellular signal transduction processes, such as secretion, gene expression, proliferation and muscle contraction (1,2), many of its substrates remain unidentified. Isozymes of PKC are subdivided into conventional PKCs (cPKC), novel PKCs (nPKC) and atypical PKCs (aPKC). PKCalpha, beta I, beta II and gamma isoforms belong to cPKC (1). When activated, cPKC isozymes phosphorylate substrates containing serine or threonine, with arginine or lysine at the -3, -2 and +2 positions, and hydrophobic amino acids at position +1 (1-3). In order to identify potential PKC substrates, we have antibodies that recognize these motifs. As shown by ELISA, Phospho-(Ser) PKC Substrate Antibody is specific to cPKC substrates containing phospho-serine. Applications: Suitable for use in ELISA, Western Blot and Immunoprecipitation. Other applications not tested. Recommended Dilution: Western Blot: 1:1000, Immunoprecipitation: 1:50, ELISA: 1:1000, Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.