Anti-phospho-Hice1 (Ser70)

Anti-Hice1 pS70 affinity-purified antibody is directed against the phosphorylated form of human Hice1 protein at the pS70 residue. The product was affinity purified from monospecific antiserum by immunoaffinity purification.  Antiserum was first purified against the phosphorylated form of the immunizing peptide.  The resultant affinity purified antibody was then cross adsorbed against the non-phosphorylated form of the immunizing peptide.  The antibody is specific for the phosphorylated form of Hice1.   Reactivity with non-phosphorylated human Hice1 is minimal by ELISA and western blot.  A BLAST analysis was used to suggest cross reactivity with Hice1 from human based on 100% sequence homology with the immunogen.  Reactivity against homologues from other sources is not known. Protein function: Contributes to mitotic spindle assembly, maintenance of centrosome integrity and completion of cytokinesis as part of the HAUS augmin-like complex. [The UniProt Consortium]