Anti-phospho-14-3-3 (Ser58)

Anti-14-3-3 pS58 antibody is directed against rat 14-3-3 protein phosphorylated at S58. The antibody was prepared from monospecific antiserum by immunoaffinity chromatography using phospho peptide coupled to agarose beads followed by solid phase adsorption(s) against non-phospho peptide and non-specific peptide to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum. This antibody is specific for phosphorylated 14-3-3. Minimal reactivity occurs against non-phosphorylated 14-3-3. Reactivity against 14-3-3 occurs from rat and human sources. Reactivity is expected against the following species based on 100% sequence homology: bovine, canine, chicken, mouse, non-human primates, sheep, Xenopus and zebra fish. Protein function: Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner. Negative regulator of osteogenesis. Blocks the nuclear translocation of the phosphorylated form (by AKT1) of SRPK2 and antagonizes its stimulatory effect on cyclin D1 expression resulting in blockage of neuronal apoptosis elicited by SRPK2. [The UniProt Consortium]