Anti-GluR1 (Phospho-Ser845)

GluR1 is a subunit of the AMPA ionotropic glutamate receptor, which is responsible for fast excitatory synaptic transmission in the CNS. AMPA receptors are composed of four subunits, GluR1, GluR2, GluR3, and GluR4, which combine into heterotetramers to form a cation-permeable pore in the plasma membrane. Each subunit has two isoforms with the primary isoform designated as flip and a second isoform generated through alternative splicing designated as flop. The GluR1 flip and flop isoforms do not affect desensitization or channel opening and closing kinetics. GluR1 can be phosphorylated by PKA at serine 845 (Ser845), which increases the peak open probability of the ion channel, and dephosphorylation is required for AMPA receptor endocytosis. GluR1 (phospho-Ser845) levels increase in the ventromedial prefrontal cortex (vmPFC) and nucleus accumbens (NAc) following cocaine-cue extinction. Levels of GluR1 (phospho-Ser845) are reduced following NMDA receptor activation in rat hippocampal slices and by amyloid-beta (Abeta) oligomers in primary mouse neurons. Cayman's GluR1 (Phospho-Ser845) Polyclonal Antibody can be used for immunohistochemistry (IHC) and Western blot (WB) applications. The antibody recognizes Glur1 (phosphor-Ser845) at approximately 100 kDa from mouse, rat, and turtle samples.Synonyms: AMPA-selective Glutamate Receptor 1, GluR-1, GluR-A, GluA1. Immunogen: Phosphopeptide corresponding to amino residues surrounding the phospho-Ser845 of rat GluR1. Formulation: (Request formulation change), Affinity-purified polyclonal antibody from pooled serum. Storage Buffer: 10 mM HEPES, pH 7.5, with 150 mM sodium chloride, 100 µg/ml BSA, and 50% glycerol. Host: Rabbit. Applications: IHC and WB. Cross Reactivity: (+) GluR1 (phosphor-Ser845), (-) Non-phosphorylated GluR1. Origin: Animal/Rabbit.