Anti-XRCC6 (X Ray Repair Complementing Defective Repair in Chinese Hamster Cells 6, X-ray Repair Com

Originally identified as an autoantigen, the Ku proteins are nuclear proteins found in eukaryotes. They exist as a heterodimer consisting of Ku70 and Ku80, and migrate at approximately 70 and 80kD, respectively. They bind to DNA ends at double-strand breaks. The Ku proteins interact with the DNA-dependent protein kinase (DNA-PK), and participate in the targeting of DNA-PK to DNA. The Ku proteins may participate in numerous cellular processes, including DNA repair and replication, cell signaling, and proliferation, transcriptional activation, and apoptosis. Applications: Suitable for use in ELISA and Western Blot. Other applications not tested. Recommended Dilutions: ELISA: 1:2000, Western Blot: 0.1-0.3ug/ml, Optimal dilutions to be determined by the researcher. Positive Control: Lysates of cell lines A431, HeLa and HepG2, Storage and Stability: May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20°C. Aliquots are stable for at least 12 monthsat -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. Peptide Blocking: Corresponding peptide is available for Peptide Blocking studies. See X9506-05J-P. Antibody is typically 0.5mg/ml and peptide is supplied as a 100ul pellet. When peptide is reconstituted in 200ul water, the concentration would also be 0.5mg/ml. To start, the best ratio would be 1:1 (molar excess of peptide relative to antibody when identical volumes are mixed). Mix equal volumes of peptide and antibody at the required dilution and leave at ambient temperature. It is best is to have two identical blots to be incubated with equal amount of antibody, but one with the antibody pre-adsorbed to the peptide for 20min. Then incubate and develop the two blots in parallel.