Anti-Wnt10B (Wnt-10b, Wnt-12)

Wnt­10b (also known as Wnt­12) is a 42­44kD member of the Wnt family of secreted, highly conserved, cysteine­rich glycoproteins that play important roles in vertebrate pattern formation, cell fate decision, axon guidance, and tumor formation (1­3). Human Wnt­10b cDNA encodes a 389 amino acid (aa) precursor that contains a 28 aa signal sequence plus a 361 aa mature protein that contains two glycosylation sites, three potential phosphorylation sites, and a potential palmitoylation site (3, 4). Human Wnt­10b shares 97­99% aa identity with mouse, rat, equine, porcine, and canine Wnt­10b. Wnt­10b plays a critical role in maintaining mesenchymal stem cells and determining whether they differentiate to adipocytes or osteoblasts (5­7). Mouse Wnt­10b deletion produces age­dependent loss of bone mass due to defective production of osteoblasts, while transgenic over­expression increases postnatal osteoblast differentiation and inhibits adipocyte differentiation (5­7). Ectopic expression of Wnt­10b in an obesity and diabetes­prone background, such as the ob/ob mouse, inhibits obesity (8). In mouse skeletal muscle, Wnt­10b is expressed inversely with SREBP1c and increases insulin sensitivity (9). In humans, a missense polymorphism is responsible for a malformation of hands and feet, while a C256Y inactivating mutation is associated with severe early­onset obesity (10, 11). Wnt­10b is mainly produced by stem cells and pre­osteoblasts, but also by adult bone marrow CD8+ T lymphocytes stimulated with parathyroid hormone (12). In some hepatocellular carcinomas, Wnt­10b can inhibit cancer cell growth, but in others, it can act synergistically with FGFs to stimulate cell growth (13). Several Wnts, including Wnt­10b, are expressed in both normal and/or malignant colon tissues (14). Applications: Suitable for use in ELISA and Immunohistochemistry. Other applications not tested. Recommended Dilution: Immunohistochemistry: 8­25ug/ml, Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.