Anti-Vinculin (VCL, VINC, CMD1W, CMH15, Metavinculin, MVCL)

Vinculin is a cytoskeletal protein associated with cell-cell and cell-matrix adherens type junctions. Multiple isoforms are produced by alternative splicing. Applications: Suitable for use in Western Blot and Immunohistochemistry. Other applications not tested. , Recommended Dilutions: Western Blot: 1-2ug/ml, Immunohistochemistry: 2-4ug/ml in formalin/acetone fixed frozen tissues., Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.