Anti-Vasoactive Intestinal Peptide (VIP)

Vasoactive intestinal peptide (VIP) is a 28aa peptide that exerts wide-ranging effects in embryonic and adult tissues through interactions with the 7-transmembrane spanning receptors VIP R1 and VIP R2. The VIP proprotein is proteolytically processed to release bioactive VIP as well as the bioactive peptides PHV and PHM. VIP regulates appetite and feeding behavior and stimulates insulin and glucagon secretion in pancreatic islet cells. It also regulates astrocyte function, multiple aspects of cardiac function, and TLR function in innate immune responses. Mature VIP shares 100% aa sequence identity with mouse and rat VIP. Applications: Suitable for use in ELISA and Immunohistochemistry. Other applications not tested. Recommended Dilutions: Immunohistochemistry: 25ug/ml detects VIP in immersion-fixed paraffin-embedded normal human and stomach cancer tissue sections., Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized powder may be stored at -20°C. Stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.