Anti-VAPA (Vesicle-associated Membrane Protein-associated Protein A, VAMP-A, VAMP-associated Protein

VAP-A (VAMP-associated protein A, also VAP-33) is a 33kD integral membrane protein that is ubiquitously expressed and found associated with multiple cellular organelles, including the Golgi, endosomes, tight junctions, and the cell membrane. VAP-A appears to be pleiotrophic in function, participating in synaptic vesicle fusion, maintenance of tight junction integrity, and the regulation of cholesterol synthesis. Human VAP-A is a type IV (small luminal domain) transmembrane protein 249aa in length. It is almost all cytoplasmic (aa1-227) with a one aa luminal fragment. The cytoplasmic region contains an MSP/motile sperm protein domain (aa14-131) plus a coiled-coil region (aa169-205). VAP-A homo- and hetero-dimerizes with VAP-B. It has multiple binding partners within the cell. There is one potential alternative start site at Met8, and an isoform that shows a 44aa insertion after Leu140. Over aa1-132, human VAP-A shares 97% aa identity with mouse VAP-A. Applications: Suitable for use in ELISA and Western Blot. Other applications not tested. Recommended Dilutions: ELISA: 0.5-1ug/ml, Western Blot: 2ug/ml, Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.