Anti-Taxilin, alpha (TXLNA) TXLN-alpha, IL­14alpha)

alpha­Taxilin (TXLN­alpha, also p75 [in rat] and IL­14alpha) is the founding member of the taxilin family of proteins. Although its predicted MW is 62kD, it runs anomalously at 74­76kD in SDS­PAGE. alpha­Taxilin is ubiquitously expressed and participates in intracellular vesicle trafficking. In particular, alpha­Taxilin interacts with syntaxin family members that localize to the plasma membrane, providing for SNARE complex formation. alpha­Taxilin also regulates the placement of growing protein chains. Human alpha­Taxilin is 546 amino acids (aa) in length. Its defining characteristic is a large coiled­coil region between aa186­491. When an alternate start site at Met176 is used, a secreted cytokine is generated termed IL­14alpha. Over aa1­162, human alpha­Taxilin shares 81% aa identity with mouse alpha­Taxilin. Applications: Suitable for use in ELISA and Western Blot. Other applications not tested. Recommended Dilution: Western Blot: 2ug/ml, Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.