Anti-Stromal Interaction Molecule 1 (STIM1)

In T lymphocytes, the sole pathway for Ca++ entry following antigen-receptor binding is through store-operated Ca++- release-activated Ca++ (CRAC) channels (reviewed in 1). These channels are made up of the pore-forming subunit ORAI1 (2) and the stromal interaction molecule 1 (STIM1), a protein that functions as a Ca++ sensor and activates the CRAC channels, migrating to the plasma membrane from endoplasmic reticulum (ER)-like sites which act as the Ca++ store (3,4). A related molecule, STIM2, acts to inhibit the STIM1-mediated store-operated Ca++ entry, and can form complexes with STIM1, suggesting they may play a, coordinated role in controlling Ca++ entry (5). At least two isoforms of STIM1 are known to exist, this antibody will detect only the larger form. This STIM1 antibody is predicted, to have no cross-reactivity to STIM2. Applications: Suitable for use in ELISA, Western Blot and Immunohistochemistry. Other applications not tested. Recommended Dilution: Western Blot: 1-2 ug/ml., Immunohistochemistry: 2.5ug/ml, Optimal dilutions to be determined by the researcher. Positive Control: Mouse thymus tissue lysate, Human spleen tissue lysate, Storage and Stability: May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile 40-50% glycerol, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.