Anti-Stabilin-1 (CLEVER-1, FEEL-1, FELE-1, FEX1, MS-1 Antigen, STAB1, Fasciclin, EGF-like, laminin t

Stabilin­1, also known as FEEL­1 (fasciclin, EGF­like, laminin type EGF­like, and link domain containing scavenger receptor­1) and CLEVER­1 (common lymphatic endothelial and vascular endothelial receptor­1), was originally identified as the MS­1 antigen. It is a type I transmembrane multi­domain protein with a large extracellular domain that contains 7 fasciclin domains, multiple EGF­like and laminin type EGF­like domains, and a link domain. The short cytoplasmic tail interacts with cargo adaptors that link cargo into the clathrin­coated pits (CGAs). Stabilin­1 is expressed by tissue macrophages and sinusoidal endothelial cells, which are scavenging cells. Stabilin­1 is involved in intracellular trafficking pathways and binds multiple ligands, including acetylated LDL, SPARC, AGE, and SI­CLP. An alternatively spliced, soluble, 803 amino acid (aa) Stabilin­1 that diverges after aa746 has been identified. Within the region used as immunogen, human Stabilin­1 shares 83% and 84% amino acid sequence homology with mouse and canine Stabilin­1, respectively. Applications: Suitable for use in ELISA and Immunohistochemistry. Other applications not tested. Recommended Dilution: Immunohistochemistry: 8­25ug/ml, Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.