Anti-Spastin (N-Term) Rabbit Monoclonal Antibody (Clone RM346)

Spastin is an ATP-dependent microtubule-severing protein and a member of the ATPases associated with various cellular activities (AAA) family encoded by SPG4 in humans. It is composed of a hydrophobic region, microtubule-interacting and endosomal trafficking (MIT) domain, microtubule-binding domain, and an AAA ATPase domain. It is ubiquitously expressed and localized to the endoplasmic reticulum. Alternative splicing of SPG4 produces a second shorter isoform that lacks the first 87 amino acids of full-length spastin and is localized to the cytosol. Spastin functions as a hexamer that binds to tubulin dimers on the plus-end of microtubules and disrupts tubulin dimer interactions in an ATP-dependent manner to sever them from microtubules. Mutations in SPG4 are associated with autosomal dominant uncomplicated hereditary spastic paraplegia (HSP). Cayman's Spastin (N-Term) Rabbit Monoclonal Antibody (Clone RM346) can be used for immunohistochemistry (IHC) and Western blot (WB) applications.Synonyms: Spastic Paraplegia 4, SPG4. Immunogen: Peptide from the N-terminal region of human spastin. Clone Designation: RM346. Formulation: (Request formulation change), 100 µl of protein A-affinity purified monoclonal antibody. Storage Buffer: PBS with 50% glycerol, 1% BSA, and 0.09% sodium azide. Host: Rabbit. Isotype: IgG. Applications: IHC and WB. Cross Reactivity: (+) Spastin.