Anti-SOX17 (SRY-box containing gene, Sex determining region Y-box 17, SRY) (NL557)

SOX17 is a member of the SOX family of transcription factors that bind DNA via a high mobility group (HMG) domain. SOX17 is suggested to play an important role in endoderm development. Applications: Suitable for use in Immunocytochemistry. Other applications not tested. Recommended Dilutions: Immunocytochemistry: 1:10: Tested using definitive endoderm differentiated BG01V cells. Cells were fixed in PBS containing 4% paraformaldehyde, and blocked with PBS containing 10% normal donkey serum,0.1% Triton(R) X-100, and 1% BSA. After blocking, cells were incubated with S5364-45A (1:10) in blocking buffer for 3 hours in the dark. Between each step, cells were washed with PBS containing BSA. If a staining volume of 20 tests can be done in a staining volume of 250ul, 100 tests can be done in a staining volume of 50ul., Optimal dilutions to be determined by the researcher. Absorption: 557nm , Emission: 574nm , Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. NorthernLights 557 conjugates are sensitive to light.