Anti-Set1

Make to order. Production time: 70-80 business days. Deliverables: 1. 200 µg antigen (used as positive control), 2. 1 ml pre-immune serum (used as negative control) , 3. Rabbit polyclonal antibody, antigen affinity purified. Quality Guarantee: 1. Antibody purity > 90% confirmed by SDS-PAGE, 2. Antibody titer > 1: 64,000 confirmed by ELISA, 3. WB positive with the immunogen protein (not applicable for synthetic peptide). Applications: ELISA, WB (positive for the recombinant immunogen protein/peptide). Buffer: Preservative: 0.03% Proclin 300, Constituents: 50% Glycerol, 0.01M PBS, pH 7.4. Protein function: Catalytic component of the COMPASS (Set1C) complex that specifically mono-, di- and trimethylates histone H3 to form H3K4me1/2/3 (PubMed:21694722, PubMed:21875999, PubMed:22048023). Binds RNAs which might negatively affect its histone methyltransferase activity. COMPASS recognizes ubiquitinated H2B on one face of the nucleosome which stimulates the methylation of H3 on the opposing face. Set1-dependent trimethylation regulates chromatin changes at active promoters that ensure optimal RNA polymerase II release into productive elongation, thereby contributing to optimal transcription (PubMed:21694722). [The UniProt Consortium]