Anti-Retinoblastoma (Rb, Retinoblastoma 1, Retinoblastoma 1 (Including Osteosarcoma), Rb1, Rb1 Prote

Retinoblastoma 1 protein (RB1) also retinoblastoma associated protein, pp110, and p105Rb is a 110kD tumor suppressor gene and member of the retinoblastoma protein family. Human RB1 is 928aa in length. The protein contains a Pocket domain (aa 373771 which is comprised of two other domains, domain A (aa 373573 and domain B (aa 640771and a "spacer" aa580639 The Pocket domain binds to threoninephosphorylated domain C aa771928 which thereby prevents interaction with heterodimeric E2F/DP transcription factor complexes. Human RB1 is 90% aa identical to mouse RB1. RB1 is expressed in the retina The underphosphorylated, active form of RB1 interacts with E2F1 and represses its transcription activity, leading to cell cycle arrest. Defects in RB1 lead to the childhood cancer retinoblastoma. Applications: Suitable for use in Western Blot. Other applications not tested. Recommended Dilution: Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.