Anti-RAB5A (Ras-related protein Rab-5A)

RAB5A(Ras-associated protein RAB5A), also called RAB5, is a protein that in humans is encoded by , the RAB5A gene. RAB5 is a rate-limiting component of the machinery regulating the kinetics of , membrane traffic in the early endocytic pathway. The RAB5A gene is mapped on 3p24.3. Tuberin , functions as a RAB5GAP in vivo to negatively regulate RAB5-GTP activity in endocytosis. Ectopically , expressed ALS2 localized with RAB5 and early endosome antigen-1 onto early endosomal , compartments and stimulated the enlargement of endosomes in cultured cortical neurons. RAB5 is , indispensable for a form of receptor tyrosine kinase-induced actin remodeling called circular , ruffling. RAB5 signals to the actin cytoskeleton through RNTRE, a RAB5-specific GTPase-activating , protein (GAP). RAB5 activity on phagosome membranes began to increase on disassembly of the actin , coat encapsulating phagosomes. RAB5 activation was either continuous or repetitive for up to 10 , minutes, but it ended before the collapse of engulfed apoptotic cells. Expression of a dominant-negative , mutant of RAB5 delayed this collapse of apoptotic thymocytes, showing a role for RAB5 in phagosome , maturation. UniProt Number: P20339, Gene ID: RAB5A, Applications: Suitable for use in Western Blot and Immunohistochemistry (Paraffin). Recommended Dilution: Optimal dilutions to be determined by the researcher. Storage and Handling: Store at -20?C for one year. After reconstitution, store at 4?C for one month. Can also be aliquoted and stored frozen at -20?C for long term., Avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.