Anti-PUM1 (Pumilio Homolog 1, HSPUM, PUMH1, Pumilio-1, PUML1)

PUM1 (Pumilio homolog 1/Pumilio-1) is a 130-140kD member of the PUF (PUmilio and FBF) family of RNA-binding proteins. It is ubiquitously expressed, and appears to be involved in cell cycle regulation. Among other things, PUM1 is known to bind to the 3' UTR of p27 mRNA following cell activation. This results in a conformational change in the p27 transcript, allowing for miRNA binding and translational repression, followed by a decline in p27 availability and entry into S-phase of the cell cycle. Human PUM1 is 1186 amino acids (aa) in length. It contains N-terminal Ala-, Gln- and Ser-rich regions (aa393-815), one PUM-HD region (aa828-1168) and eight consecutive pumilio repeats (aa726-1142). There are six utilized Ser/Thr phosphorylation sites, and at least three isoform variants. One contains a five aa insert after Gly418, a second possesses an alternative start site 36aa upstream of the standard site, and a third shows a deletion of aa597-623 coupled to a two aa insertion after Gln950. Over aa230-372, human PUM1 is identical to mouse PUM1 in amino acid sequence. Overall, human and mouse PUM1 share 99% aa sequence identity. Applications: Suitable for use in ELISA, Western Blot, Immunohistochemistry. Other applications not tested. Recommended Dilution: Western Blot: 1ug/ml, Immunocytochemistry: 5-15ug/ml, Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile ddH2O or PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.