Anti-Protein S

Protein S is a 69kD, secreted glycoprotein that belongs to a general group of vitamin K-dependent proteins. It is synthesized principally by hepatocytes, circulates both free (40%) and bound to C4bBP (60%), interacts with protein C to suppress coagulation, and binds TAM family RTKs during immune regulation. Mature human Protein S is 634 amino acids in length. It contains one Gla domain (aa42-87), four EGF-like repeats (aa117-283) and two laminin G-like domains (aa299-666). Over aa42-670, human Protein S shares 83%, 80%, and 79% aa sequence identity with porcine, mouse and canine Protein S, respectively. Applications: Suitable for use in Western Blot and ELISA. Other applications not tested. Recommended Dilution: Western Blot: 0.1-0.2ug/ml. The detection limit is ~5ng/lane under non-reducing and reducing conditions., ELISA (dierct): 0.5-1ug/ml. The detection limit is ~0.2ng/well. , Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute by adding sterile 40-50% glycerol, PBS. Aliquot and store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.